DR.HYTHAM KADOUR

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 اقرأ المزيد عن التريبسين

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مُساهمةموضوع: اقرأ المزيد عن التريبسين   الثلاثاء فبراير 26, 2008 3:43 am

Product Contents
Sequencing Grade Modified Trypsin:
Part No. Size
V511A 100μg
(5 × 20μg)
Description: Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Unmodified
trypsin is subject to proteolysis, generating fragments that can interfere with protein sequencing or HPLC peptide analysis.
In addition, proteolysis can result in the generation of pseudotrypsin, which has been shown to exhibit chymotrypsin-like
specificity (1). Promega Sequencing Grade Modified Trypsin is porcine trypsin modified by reductive methylation, rendering it
resistant to proteolytic digestion (2). In enzymatic stability tests, modified trypsin was found to retain greater than two times the
activity of unmodified trypsin.
Sequencing Grade Modified Trypsin is further improved by TPCK treatment followed by affinity purification, yielding a highly
active and stable molecule. Sequencing Grade Modified Trypsin is provided in 20μg aliquots with a stability-optimized
resuspension buffer. A protease:protein ratio of 1:100 to 1:20 (w/w) is recommended for protein sequencing.
Physical Form: Sequencing Grade Modified Trypsin is supplied lyophilized.
Resuspension Buffer (supplied): Trypsin Resuspension Buffer (V542A), provided with this product, is composed of 50mM
acetic acid.
Specific Activity: See Product Information Label.
Storage Conditions: Store the lyophilized powder at –20°C. Store reconstituted enzyme at –70°C. Thaw the reconstituted
trypsin at room temperature, placing on ice immediately after thawing. Remove the amount of trypsin needed, then refreeze
the unused portion. To maintain maximum product activity, limit the number of freeze-thaw cycles to five. See the product
label for the expiration date.
Unit Definition: One unit is the amount of Sequencing Grade Modified Trypsin required to produce a ΔA253 of 0.001 per
minute at 30°C with the substrate α-benzoyl-L-arginine ethyl ester (BAEE). The substrate is dissolved in 50mM Tris-HCl
(pH 7.6), 1mM CaCl2, and the enzyme is diluted in 50mM acetic acid.
Usage Notes:
1. For maximum activity, resuspend Sequencing Grade Modified Trypsin in the Trypsin Resuspension Buffer provided, and
heat at 30°C for 15 minutes before use.
2. Specific activities may vary widely between suppliers. Procedures written for use of trypsin by weight may need to be
optimized based on enzyme activity.
Quality Control Assays
Stability: A 0.1mg/ml solution of Sequencing Grade Modified Trypsin retains at least 85% of its activity after a 3-hour
incubation at 37°C in 40mM NH4HCO3.
Sequence Specificity: Fifty micrograms of insulin β-chain are incubated with 2.5μg of Sequencing Grade Modified Trypsin
for 2 hours and for approximately 18 hours at 37°C. The digestion products are separated by reverse phase HPLC and detected
at 215nm. The minimum passing specifications are that the 18-hour digest shows the two main digestion products and has no
significant new peaks compared with the 2-hour digest.
Promega Corporation
2800 Woods Hollow Road
Madison, WI 53711-5399 USA
Telephone 608-274-4330
Toll Free 800-356-9526
Fax 608-277-2516
Internet www.promega.com
PRODUCT USE LIMITATIONS, WARRANTY, DISCLAIMER
Promega manufactures products for a number of
intended uses. Please refer to the product label for the
intended use statements for specific products.
Promega products contain chemicals which may be
harmful if misused. Due care should be exercised with
all Promega products to prevent direct human contact.
Each Promega product is shipped with documentation
stating specifications and other technical information.
Promega products are warranted to meet or exceed the
stated specifications. Promega's sole obligation and the
customer's sole remedy is limited to replacement of
products free of charge in the event products fail to perform
as warranted. Promega makes no other warranty
of any kind whatsoever, and SPECIFICALLY DISCLAIMS
AND EXCLUDES ALL OTHER WARRANTIES
OF ANY KIND OR NATURE WHATSOEVER, DIRECTLY
OR INDIRECTLY, EXPRESS OR IMPLIED, INCLUDING,
WITHOUT LIMITATION, AS TO THE SUITABILITY, PRODUCTIVITY,
DURABILITY, FITNESS FOR A PARTICULAR
PURPOSE OR USE, MERCHANTABILITY, CONDITION,
OR ANY OTHER MATTER WITH RESPECT TO
PROMEGA PRODUCTS. In no event shall Promega be
liable for claims for any other damages, whether direct,
incidental, foreseeable, consequential, or special
(including but not limited to loss of use, revenue or
profit), whether based upon warranty, contract, tort
(including negligence) or strict liability arising in connection
with the sale or the failure of Promega products
to perform in accordance with the stated specifications.
Part# 9PIV511
Revised 4/07
Part# 9PIV511
Printed in USA. Revised 4/07
© 1998–2007 Promega Corporation. All Rights
Reserved.
All specifications are subject to change without prior
notice.
Product claims are subject to change. Please contact
Promega Technical Services or access the Promega
online catalog for the most up-to-date information on
Promega products.
AF9PI V511 0407V511
I. Product Information
A. Specificity
Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine
and arginine. Restrictions to the specificity of trypsin occur when proline is at the
carboxylic side of lysine or arginine; the bond is almost completely resistant to
cleavage by trypsin. Cleavage may also be considerably reduced when acidic
residues are present on either side of a potentially susceptible bond (3).
B. Stability
Modified trypsin is maximally active in the pH range of 7–9 and reversibly inactivated
at pH 4. It is resistant to mild denaturing conditions: 0.1% SDS, 1M urea, or
10% acetonitrile (4). Modified trypsin retains 48% activity in 2M guanidine HCl (3).
II. Protocol
A. Protein Denaturation
In general, proteins require denaturation and disulfide bond cleavage before
enzymatic digestion can go to completion (3).
Dissolve 1–10mg of the target protein in 6M guanidine HCl (or 6–8M urea), 50mM
Tris-HCl (pH Cool, 2–4mM DTT (or β-mercaptoethanol) in a reaction volume of up to
1ml (25μl minimum). Heat at 95°C for 15–20 minutes or at least 60°C for 45–60
minutes. If smaller amounts of protein are to be digested, the recommended conditions
given can be scaled down proportionally. However, under no conditions should
less than 25μl of dissolving agent be used.
After denaturation, allow the reaction to cool and add 50mM NH4HCO3 (pH 7.Cool or
50mM Tris-HCl, 1mM CaCl2 (pH 7.6), until the guanidine-HCl or urea concentration
is below 1M.
B. Protease Digestion
Add modified trypsin to a final protease:protein ratio of 1:100 to 1:20 (w/w). Incubate
at 37°C for at least 1 hour. Remove a small aliquot and chill the reaction on ice or
freeze. Add an inhibitor to the aliquot to terminate the protease activity or precipitate
the sample by the addition of TCA to a 10% final concentration. Determine the extent
of digestion by subjecting a portion of the digestion products to reverse phase HPLC
or SDS-PAGE. If further proteolysis is required, return the reaction tube to 37°C and
continue incubating until the desired digestion is obtained (5). The reaction can be
terminated by freezing or by the addition of specific inhibitors. Trypsin can also be
inactivated by lowering the pH of the reaction to below 4. Trypsin will regain activity
as the pH is raised above 4 (3). Reducing the temperature will decrease the digestion
rate. Longer incubations, up to 24 hours, may be required depending on the nature of
the protein. If using long incubations, be very careful to avoid bacterial contamination.
If a partial digestion of a nondenatured substrate is desired, as would be necessary for
analysis of the domain structure of a protein, incubate the protein with modified
trypsin at a protease:protein ratio of 1:100 to 1:20 in a buffer compatible with the
stability of the target protein.
III. Related Products
Product Size Cat.#
Proteinase K 100mg V3021
For Laboratory Use.
IV. References
1. Keil-Dlouha, V. (1971) Proteolytic activity of pseudotrypsin. FEBS Lett. 16, 291–95.
2. Rice R.H. et al. (1977) Stabilization of bovine trypsin by reductive methylation.
Biochem. Biophys. Acta 492, 316–21.
3. Wilkinson, J.M. (1986) “Fragmentation of Polypeptides by Enzymic Methods”. In:
Practical Protein Chemistry: A Handbook. A. Darbre, ed., John Wiley and Sons, New
York, N.Y.
4. Bond, J.S. (1989) “Commercially Available Proteases”, Appendix II. In: Proteolytic
Enzymes, A Practical Approach. R.J. Beynon and J.S. Bond, eds., IRL Press, Oxford,
U.K.
5. Flannery, A.V., Beynon, R.J. and Bond, J.S. (1989) “Proteolysis of Proteins for
Sequencing Analysis and Peptide Mapping”. In: Proteolytic Enzymes: A Practical
Approach. R.J. Beynon and J.S. Bond, eds., IRL Press, Oxford, U.K.
Usage Information
Promega Corporation · 2800 Woods Hollow Road·Madison, WI 53711-5399 U.S.A. · Toll Free in the USA 800-356-9526 · Telephone 608-274-4330 · Internet www.promega.com
Part# 9PIV511
Printed in USA. Revised 4/07
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